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. 2011 Nov 23;287(3):1847–1860. doi: 10.1074/jbc.M111.302356

FIGURE 3.

FIGURE 3.

Effect of fasting and exercise on IL1Rn, IL15Rα, and Tweak mRNA and IL1Rn protein expression in rat livers. A, animals were fasted for 24 or 48 h and sacrificed. Livers were isolated immediately and snap frozen in liquid nitrogen. Random liver sections of a minimum of 80 mg were used for mRNA isolation. mRNA expression was measured with SYBR Green qPCR or TaqMan probes (IL1Rn and Tweak). Values are presented relative to the mean of control sample (Fed) for each time point (Student's two-tailed t test; **, p < 0.01; ***, p < 0.001). B, IL1RN was detected in cytosolic protein fraction of random rat liver sections of a minimum of 80 mg. Animals in the experimental group were fasted for 48 h. Proteins were separated in 15% SDS-PAGE gel, and β-actin was used as a loading control. Migration of molecular weight markers is indicated. C, animals were forced to run once or twice for 1 h each time and sacrificed as specified. mRNA was isolated and measured as stated above. Hours represent time after the first exercise period. Values are presented relative to the mean of corresponding control samples (Not Exercised) (Student's two-tailed t test; *, p < 0.05; **, p < 0.01). Error bars, S.D.