FIGURE 4.

Effect of PGC-1α-Ad on IL1Rn, IL15Rα, and Tweak is mediated by a nuclear receptor. A, mouse primary hepatocytes were transduced with wild type (WT) or L2L3M mutant PGC-1α-Ad or GFP-Ad at MOI = 1. Cells were collected after 48 h, and mRNA expression was analyzed with SYBR Green qPCR. Data are presented as -fold change compared with GFP-Ad controls. Values represent means ± S.D. (error bars) (n = 4). ***, p < 0.001 statistical significance compared with GFP-Ad samples. (Student's two-tailed t test). B, cells were treated as described (Fig. 1B), and expression of PPARα was measured with qPCR. Data are presented as -fold change compared with uninfected controls at a given time point. Values represent means ± S.D. (n = 3). ***, p < 0.001, statistical significance compared with GFP-Ad samples, Student's two-tailed t test. C, mouse primary hepatocytes were transfected with the Il1rn upstream promoter luciferase construct and expression vectors as indicated. Cells were collected 48 h later, and luciferase activity was measured. The values are presented relative to pcDNA3 control and represent means ± S.D. *, statistical significance compared with pcDNA3 control samples (Student's two-tailed t test; *, p < 0.05; **, p < 0.01; #, p < 0.05; n = 10–12/point). D, PGC-1α-Ad effect involves PPARα. Cells were transfected with 150 pmol/μl of control (Scramble) or PPARα siRNA before transduction with PGC-1α-Ad and collected after 48 h. mRNAs were measured with SYBR Green qPCR. Values are presented relative to the control (Scramble) sample and represent means ± S.D. (n = 3). **, p < 0.01; ***, p < 0.001 compared with the control (Student's two-tailed t test).