TABLE 1.

Kinetic parameters of PA0743 with various substrates

Protein and variable substrate	Km	Vmax	kcat	kcat/Km
	mm	μmol/min mg protein	s−1	m−1s−1
Wild type
l-Serine	2.5 ± 0.1	18.7 ± 0.3	10.4 ± 0.2	0.4 × 104
Methyl-dl-serine	2.4 ± 0.2	17.2 ± 0.4	9.6 ± 0.2	0.4 × 104
dl-Glyceric acid	19.8 ± 0.4	10.4 ± 0.1	5.8 ± 0.1	0.3 × 103
MDHPa	17.4 ± 1.2	20.9 ± 0.7	11.6 ± 0.4	0.7 × 103
NAD+	3.4 ± 0.2	2.8 ± 0.1	1.6 ± 0.1	0.5 × 103
T96A
l-Serine	12.3 ± 1.8	2.6 ± 0.2	1.4 ± 0.1	0.1 × 103
S122A
l-Serine	10.6 ± 0.5	0.8 ± 0.02	0.4 ± 0.01	0.4 × 102
N175A
l-Serine	4.2 ± 0.2	4.9 ± 0.1	2.7 ± 0.1	0.6 × 103
Y219A
l-Serine	11.0 ± 2.4	1.4 ± 0.1	0.8 ± 0.1	0.7 × 102

^a Methyl-2,2-dimethyl-3-hydroxypropionate.