TABLE 4.

The measured k_on, k_off, and K_D values of M. avium subsp. paratuberculosis Ag85 bound to Fn

Values represent the mean ± S.E.

	Fn-binding motifa	kon	koff	KD
		m−1s−1	s−1	nm
MAP Ag85A	FEWYNQSGISV	9.2 × 104 ± 1.1	6.3 × 10−3 ± 0.8	68.4 ± 4.6
MAP Ag85B	FEWYYQSGLSV	1.2 × 105 ± 0.2	4.4 × 10−3 ± 0.7	36.7 ± 5.4
MAP Ag85C	FEEFYQSGLSV	1.4 × 104 ± 0.3	4.7 × 10−3 ± 0.7	33.6 ± 4.2

^a Data were reported by Naito et al. (23).