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. 2011 Nov 29;287(3):1903–1914. doi: 10.1074/jbc.M111.256586

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FIGURE 5. — PA3347 interacts with FlgM and PA3346C. A, interaction between PA3347 and FlgM. Lane 1, molecular mass markers; lane 2, P. aeruginosa lysate; lane 3, His₆-FlgM; lane 4, P. aeruginosa lysate incubated with His₆-FlgM; lane 5, E. coli lysate expressing GST-PA3347-S56A; lane 6, His₆-FlgM; lane 7, E. coli lysate expressing GST-PA3347-S56A incubated with His₆-FlgM. B, interaction between PA3347 and PA3346C. Lane 1, molecular mass markers; lane 2, P. aeruginosa lysate; lane 3, His₆-PA3346C; lane 4, P. aeruginosa lysate incubated with His₆-PA3346C; lane 5, E. coli lysate expressing GST-PA3347-S56A; lane 6, His₆-PA3346C; lane 7, E. coli lysate expressing GST-PA3347-S56A incubated with His₆-PA3346C. IP, immunoprecipitation; WB, Western blot.