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. 2011 Nov 29;287(3):1932–1945. doi: 10.1074/jbc.M111.283457

FIGURE 5.

FIGURE 5.

Box1 motif in RET juxtamembrane domain is necessary and sufficient for the higher RET surface levels compared with TrkB. A, sequences of the juxtamembrane regions of RET and TrkB were aligned and three motifs (Box1–3) in the juxtamembrane region were divided. B, surface levels of chimeric RET-GFP receptors with Box1–3 substitution of TrkB were measured using a ratiometric fluorescence assay in transfected PC12 cells. Relative surface levels of each chimera were normalized to that of RET-GFP. The results are represented as mean ± S.E. from three independent experiments (**, p < 0.01 versus RET surface levels; one-way ANOVA). C, surface levels of chimeric TrkB receptors with Box1–3 substitution of RET were analyzed as in B. Relative surface levels of each chimera were normalized to that of TrkB-GFP. The results are represented as mean ± S.E. from three independent experiments (**, p < 0.01 versus TrkB surface levels; one-way ANOVA). D, represented images of immunostained PC12 cells used for statistics in B and C are shown. E, chimeric receptor surface levels were quantified by biotinylation methods in transfected PC12 cells. Relative surface expression levels of each chimera were normalized to that of RET-GFP. The results are represented as mean ± S.E. from three independent experiments (**, p < 0.01 versus RET surface levels; ##, p < 0.01 versus TrkB surface levels; one-way ANOVA).