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Alignment of Y194F-Δ270 (green), T82M-Δ270 (red), and D159S/Y194F-Δ270 (purple) glycogenin mutants with wild-type apo-Δ270 enzyme (blue). The structures were superimposed with the LSQKAB program (23), and the figure was prepared using the CCP4MG program (24).