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Gel filtration of glycogenin monomer, homodimer, and heterodimers. Monomeric apo-Δ270 (0.1 μm) was labeled in a 5-min incubation with 10 μm UDP-[14C]glucose and passed through a Superdex 75 column before (○) and after mixing and equilibration for 20 min with 20 μm T82M-Δ270 (■), Y194F-Δ270 (▴), D159S/Y194F-Δ270 (●), or albumin (△). 14C-Glucosylated dimeric apo-Δ270 (20 μm; □) was also analyzed.
