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Heterodimer intrasubunit autoglucosylation. Shown is the MSAE of monomeric apo-Δ270 and WT-Δ270 glycogenins before (black bars) and after (white bars) dimerization with D159S/Y194F-Δ270. The double mutant (3.96 μm) was mixed and equilibrated with 0.03 μm apo-Δ270 or WT-Δ270 and subjected to 14C autoglucosylation as described under “Experimental Procedures.”
