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. 2011 Nov 29;287(3):1962–1969. doi: 10.1074/jbc.M111.305615

FIGURE 4.

FIGURE 4.

USP19 stabilizes HIF-1α independent of ER localization. A, graphs illustrating HeLa cells, the melanoma cell line M2, and HEK293T cells overexpressing Myc-USP19, Myc-USP19(C506S), and Myc-USP19ΔTM, which lacks ER localization. The number of cells accumulating HIF-1α in USP19-overexpressing cells was scored by counting cells positive for HIF-1α immunostaining. B, Western blot of HeLa cells transiently overexpressing Myc-USP19, Myc-USP19(C506S), and Myc-USP19ΔTM in normoxia. The blots were probed against Myc, HIF-1α, and β-actin as indicated.