Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 6;287(3):2107–2118. doi: 10.1074/jbc.M111.322644

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — KAP1 binds to other DNA methyltransferases. A, Myc-tagged DNMT1 was co-expressed with FLAG-tagged KAP1 or KAP1 deletion mutants in COS cells. The monoclonal antibody against the FLAG epitope was used for immunoprecipitation, and the monoclonal antibody against the MYC epitope was used to probe the immunoprecipitate. Lane 1, wild-type KAP1; lane 2, vector only; lane 3, wild-type KAP1; lane 4, KAP1ΔBCC; lane 5, KAP1ΔHP1; lane 6, KAP1ΔPhD-BRM. B, FLAG-tagged DNMT1 was co-expressed with Myc-tagged KAP1 in COS cells. The monoclonal antibody against the FLAG epitope was used for immunoprecipitation, and the monoclonal antibody against the Myc epitope was used to probe the immunoprecipitate. Lane 1, wild-type DNMT1; lane 2, vector only. C, FLAG-tagged DNMT3L was co-expressed with Myc-tagged KAP1 in COS cells. The monoclonal antibody against the FLAG epitope was used for immunoprecipitation, and the monoclonal antibody against the Myc epitope was used to probe the immunoprecipitate. Lane 1, vector only; lane 2, FLAG::DNMT3L. D, FLAG-tagged DNMT3a (lanes 1–3) or HP1a (lanes 4–6) was co-expressed with Myc-tagged KAP1 or KAP1 deletion mutants. The monoclonal antibody against the FLAG epitope was used for immunoprecipitation, and the monoclonal antibody against the Myc epitope was used to probe the immunoprecipitate. Lane 1, KAP1ΔRBCC; lane 2, wild-type KAP1; lane 3, KAP1ΔHP1; lane 4, KAP1ΔHP1; lane 5, wild-type KAP1; lane 6, KAP1ΔRBCC.