TABLE 1.
Oligonucleotides used for PCR to generate deletion mutants of KAP1 and ZFP57
| Deletion mutant | Oligonucleotide name | Oligonucleotide sequence |
|---|---|---|
| KAP1ΔRING | KAP1-RING-FH | tgcaagcttcagtgctactccaaagac |
| KAP1ΔRING | huKAP1-RING-RH | caaagcttcagcagctccagcgcctcgg |
| KAP1ΔBCC | KAP1-RBCC-FH | gtgaagcttgtggagcctcatggtgagatg |
| KAP1ΔBCC | KAP1-BCC-RH | agaaagcttactgccactatcacg |
| KAP1ΔRBCC | KAP1-RBCC-FH | gtgaagcttgtggagcctcatggtgagatg |
| KAP1ΔRBCC | huKAP1-RING-RH | caaagcttcagcagctccagcgcctcgg |
| KAP1ΔHP1 | KAP1-HP1-FH | ctgaagcttacctctgacagccagccac |
| KAP1ΔHP1 | KAP1-HP1-RH2 | aaaagcttacttacctctccctcacc |
| KAP1ΔPhD | KAP1-PhD-FH | ctaaagcttgaagatggaagcctcagc |
| KAP1ΔPhD | KAP1-PhD-RH | ggtaagcttaggagccaccacctc |
| KAP1ΔBRM | KAP1-BRM-FH | accaagctttctgctgtgctggtag |
| KAP1ΔBRM | huKAP1-BRM-RH | gaaagcttggccaccacgccag |
| KAP1ΔPhD-BRM | KAP1-BRM-FH | accaagctttctgctgtgctggtag |
| KAP1ΔPhD-BRM | KAP1-PhD-RH | ggtaagcttaggagccaccacctc |
| ZFP57ΔKRAB | KRAB-SPHF | ttcgcatgcgaagcaagaagaaacctcaagaac |
| ZFP57ΔKRAB | KRAB-SPHR | acagcatgcgtgtcctggatggctgggaag |