FIGURE 1.

IFN-γ pretreatment does not alter EGF-induced EGFr phosphorylation but increases EGFr expression at both mRNA and protein level in T₈₄ cells. a, EGFr was immunoprecipitated, and phosphotyrosine or total EGFr was sequentially blotted in immunoprecipitates from T₈₄ cells in response to EGF stimulation at the indicated time points. Cells were pretreated for 48 h with IFN-γ or medium. A representative Western blot is shown. b, densitometric analysis (n = 8) showed a significant increase of phosphorylated EGFr in controls at 15 min of EGF stimulation. IFN-γ did not affect EGF-induced EGFr phosphorylation in T₈₄ cells. Data are expressed as percentage of control without EGF stimulation. Asterisks denote significant differences from control (*, p ≤ 0.05). c, EGFr mRNA expression in response to IFN-γ treatment over 48 h, normalized to GAPDH (n = 6, performed in triplicate). d, representative Western blot of whole cell lysates, sequentially probed for total EGFr or lamin A/C as loading control. e, densitometric analysis reveals significantly increased EGFr expression in IFN-γ-treated cells at the indicated time points with EGF stimulation. Data are expressed as -fold control. Asterisks denote significant differences from control (*, p ≤ 0.05; **, p ≤ 0.01). Error bars, S.E.