FIGURE 3.
The expression of hypothalamic Ahi1 and 5-HT2CR was changed by fasting. A, Western blot showed that the protein levels of Ahi1 were significantly increased after fasting mice for 24 or 48 h. B, real-time PCR demonstrated that the transcription of hypothalamic Ahi1 mRNA was also stimulated by fasting. C, 5-HT2CR located on the neuronal membrane under the conditions of fasting. When the expression of hypothalamic Ahi1 was up-regulated by fasting, the level of 5-HT2CR on the membrane of hypothalamic neurons was significantly reduced. D, up-regulation of hypothalamic Ahi1 induced by fasting was significantly inhibited by mCPP pretreatment. Data are expressed as mean ± S.E. *, statistically different from control group. ACTB, β-actin.