Figure 2.

ArfGAP1 and ArfGAP2 facilitate deformation of LUVs containing myrArf1•GTP, coatomer and p25 cargo peptide. LUVs were formed and incubated with the proteins and peptide as described in Figure 1. The combination of ArfGAP, myrArf1•GTP⎳S, palmitoylated p25 and coatomer are indicated on the figure. Concentrations of each are the same as in Figure 1. LUVs were stained with uranyl acetate and examined by transmission electron microscopy (TEM).