Figure 6.

Effect of ArfGAP1 and mutants on cell ultrastructure. HeLa cells were cotransfected with plasmids directing the expression of the indicated ArfGAP1 and with a plasmid for the expression of Lac Z. The cells were prepared for TEM as described in “Materials and Methods.” Random sections were examined and cells containing crystallized produced of the LacZ reaction, indicating transfection with LacZ, were analyzed. The % of cells with patches of vesicles was determined and is presented in (A). At least 24 cells were examined under each condition. Representative images of vesicle accumulation are shown in (B). Patches of vesicles are indicated by arrows. Tubular-vesicular accumulations are indicated by an arrowhead. High magnification images of the boxed areas are shown in the panels to the right.