Figure 2.

Rab12 is not involved in iron-induced TfR degradation. MEF cells transfected with control siRNA or Rab12 siRNA were treated with 50 µg/ml FAC (ferric ammonium citrate, an iron supplier) for the indicated times. Cell lysates were analyzed by 10% SDS-PAGE followed by immunoblotting with anti-Rab12 antibody (top part), anti-TfR antibody (middle part), and anti-β-actin antibody (bottom part). The positions of the molecular mass markers (in kDa) are shown on the left.