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. 2012 Jan 23;196(2):213–221. doi: 10.1083/jcb.201108175

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© 2012 Gerl et al.

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Figure 4. — IFV purification. Polarized MDCK cells were infected with IFV, and virus particles were collected after 24 h. (A) A pure virus preparation was obtained by gradient centrifugations and was analyzed by SDS-PAGE and silver staining. (B) Electron micrograph of the virus preparation with negative stain. The inset shows a higher magnification of the same preparation. Molecular markers are given in kilodaltons. NP, influenza nucleoprotein.