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CGB7 expression increases after induction of p53 in a trophoblast-enriched cell population. Cells were treated with doxorubicin for 48 h (doxo). (A) p53 protein was detected by protein gel blot. β-actin served as loading control. (B) CGB protein levels in the cell culture supernatant were measured by an immunoassay detecting all isoforms (mean ± SD, n = 3). (C) mRNA levels of CGB isoforms were determined by real-time RT-PCR with isoform-specific primers (mean ± SD, n = 4). **p < 0.01.
