Figure 2.

Induction of CGB7 expression in a primary trophoblast cell population depends on p53. A cell population enriched in primary trophoblasts was transfected with p53-directed or control siRNA. Starting 48 h after transfection cells were treated with doxorubicin for further 48 h (doxo). (A) p53 and β-actin proteins were detected by immunoblot. (B) CGB protein levels in culture media were measured by immunoassay (mean ± SD, n = 3). (C) Levels of CGB mRNAs were determined by real-time RT-PCR (mean ± SD, n = 5). Cyclin B2 expression was monitored as control (n = 3). *p < 0.05, **p < 0.01.