Figure 5.

Identification of a p53 response element in the CGB7 promoter. Dual luciferase-reporter assays were performed in SaOS-2 cells after cotransfection of wild-type, shortened or mutant CGB7 promoter-reporter constructs together with plasmids expressing wild-type or mutant p53 and a Renilla luciferase transfection control plasmid. Fold induction by p53 is displayed in each case. The CGB7 promoter constructs are numbered relative to the transcriptional start site. Empty firefly reporter vector served as control. Both half-sites of the p53 binding site (indicated in bold) in the CGB7 promoter construct −1,200/365 were mutated separately (mut 1 or mut 2) or together (mut 1 + 2). Mutant bases are in lower case italics (mean ± SD, n = 3, *p < 0.05, **p < 0.01).