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p53 binds to the p53 response element in the CGB7 promoter. Electrophoretic mobility shift assays (EMSA) using wild-type CGB7 promoter probes or probes in which the p53 response element was mutated were performed with in vitro-transcribed and -translated p53 protein and anti-p53 antibody. p21 probes (left) served as positive control.
