Figure 7.

In vivo p53 binding to a p53 response element in the CGB7 promoter. (A) Chromatin immunoprecipitation (ChIP) experiments were performed in tet-off inducible DLD-1 cells, carrying a p53 wild-type (p53wt) transgene, before and after induction of p53wt expression. (B) ChIP experiments in HCT116 cells with wild-type p53 (HCT116 p53^+/+) or with targeted deletions in both p53 alleles (HCT116 p53^−/−) 0 h, 24 h or 48 h after doxorubicin (doxo) treatment. CGB7 promoter fragments were amplified in the input (i), in a control preparation without antibody (no) and after immunoprecipitation with p53 antibody (p53) and separated on an agarose gel.