Figure 2.

Relative abundance of Fusobacterium in tumor versus normal colorectal carcinoma biopsies. Relative amounts of Fusobacterium DNA were determined between tumor and matched normal biopsies in 99 subjects, using quantitative real-time PCR (qPCR). The cycle threshold (Ct) values for the normal samples had a Ct range of 25.5 to 40, and the Ct range for the tumor samples was between 21.4 and 40. The data shown are mean values from two independent experiments. Fusobacterium load, as determined by qPCR, was found to be significantly higher in the tumor samples versus the matched control samples (two-tailed ratio t-test, p = 2.52 × 10⁻⁶).