CXC-chemokine mRNA expression of WT and ΔF508 CF mTECs, before and after stimulation with P. aeruginosa PAO1. (a) Microarray analyses of candidate chemokine expression compare responses of WT and ΔF508 CF mTECs (n = 3 Transwells). Means ± SEM are shown. *P ≤ 0.05. (b) Quantitative analysis of representative CXC-chemokine expression (MIP-2, KC, and LIX) in WT and ΔF508 CF mTECs, 6 and 24 hours after infection (n = 3 Transwells). Data are presented relative to uninfected mRNA concentrations, and are normalized to GAPDH mRNA. Means ± SEM are shown. No difference was detected between WT and ΔF508 mTEC chemokine expression before or after infection, using unpaired Student t tests for comparisons.