TABLE 1.
QUANTITATIVE ANALYSIS OF CXC-CHEMOKINE EXPRESSION IN WILD-TYPE MURINE TRACHEAL EPITHELIAL CELLS AFTER INFECTION WITH P. AERUGINOSA
| Chemokine | Aliases | 6 Hours | 24 Hours | 48 Hours |
|---|---|---|---|---|
| Cxcl1 | KC, GROα | 25.88† | 7.83† | 6.50† |
| Cxcl2 | MIP-2, GROβ | 32.74† | 8.79† | 7.64† |
| Cxcl4 | PF4 | 1.62 | 0.19 | 1.15 |
| Cxcl5 | LIX | 4.69† | 3.13† | 5.13† |
| Cxcl7 | PPBP | 1.93 | 5.02* | 5.34* |
| Cxcl9 | MIG | 0.46 | 1.56 | 4.80 |
| Cxcl10 | IP-10 | 6.87* | 7.44† | 3.49 |
| Cxcl11 | IP-9 | 0.39 | 0.99 | 0.46 |
| Cxcl12 | SDF1 | 1.17 | 1.08 | 1.08 |
| Cxcl13 | BLC | 0.87 | 0.57 | 0.12 |
| Cxcl14 | BRAK | 0.81 | 0.88 | 0.53 |
| Cxcl15 | Lungkine | 0.87 | 0.21 | 0.32 |
| Cxcl16 | SCYB16 | 1.22 | 1.02 | 0.93 |
Definition of abbreviations: BLC, B lymphocyte chemoattractant; BRAK, breast and kidney-expressed chemokine; Gro, growth-regulated oncogene; MIG, monokine induced by interferon-γ; PF4, platelet factor 4; PPBP, pro-platelet basic protein; SCYB16, small inducible cytokine subfamily B member 16; SDF1, stromal cell–derived factor-1.
Gene expression consistently up-regulated twofold was considered, and 114 genes were significantly increased after infection. Several related CXC-chemokines, including KC (Cxcl1), MIP-2 (Cxcl2), LIX (Cxcl5), and IP-10 (Cxcl10), were up-regulated at different times after 1 hour of stimulation with Pseudomonas aeruginosa PAO1.
*P ≤ 0.05 and †P ≤ 0.005, using single-factor ANOVA for comparisons.