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. 2011 Nov 15;10(22):3897–3911. doi: 10.4161/cc.10.22.18043

Figure 7.

Figure 7

Anchoring of NFAT5 isoforms to the PM with the AP MAP N terminus. Selected subset of confocal images of HeLa cells transiently transfected with the following GFP-tagged (green) constructs: isoforms NFAT5a/b/c, AP MAP (AA1–61) (control), AP MAP (AA1–61)-NFAT5a/b/c. Salt stress (350 mM NaCl) was applied for 1 h. Nuclei were stained with DAP I (blue). Full-size images are available onhttp://mendel.bii.a-star.edu.sg/SEQUENCES/NFAT5_2011/. APMAP (AA1–61) represent the transmembrane region of APMAP for anchoring at the PM. All NFAT5 isoforms shuttle into the nucleus upon salt stress. No shuttling is observed after anchoring them to the PM (Scale bars 10 µm).