Table 1.

Primer sequences and cycling conditions for the genes analyzed

Primer	Sequence Product	Length (bp)
ALP	F: 5′-CCA CGT CTT CAC ATT TGG TG-3′ R: 5′-AGA CTG CGC CTG GTA GTT GT-3′	196 bp
β-ACTIN	F: 5′-ATG AGG ATG CTC ACG GAG CGC GGC TAC AGC-3′ R: 5′-ACA CCA CTG TGT TGG CGT ACA GGT CTT TGC-3′	331 bp
CBFA-1	F: 5′-TGA GAG CCG CTT CTC CAA CC-3′ R: 5′-GCG GAA GCA TTC TGG AAG GA-3′	270 bp
COL-1	F: 5′-CCA AAT CTG TCT CCC CAG A-3′ R: 5′-TCA AAA ACG AAG GGG AGA T-3′	213 bp
DSPP	F: 5′-TCA CAA GGG AGA AGG GAA TG-3′ R: 5′-TGC CAT TTG CTG TGA TGT TT-3′	182 bp
DMP-1	F: 5′-CAG GAG CAC AGG AAA AGG AG-3′ R: 5′-CTG GTG GTA TCT TGG GCA CT-3′	213 bp
GAPDH	F: 5′-ACC ACA GTC CAT GCC ATC AC-3′ R: 5′-TCC ACC ACC CTG TTG CTG TA-3′	452 bp
hTERT	F: 5′-CCT CTG TGC TGG GCC TGG ACG ATA-3′ R: 5′-ACG GCT GGA GGT CTG TCA AGG TAG-3′	282 bp
OCN	F: 5′-ATG AGA GCC CTC ACA CTC CT-3′ R: 5′-CAA GGG GAA GAG GAA AGA AG-3′	377bp
OPN	F: 5′-CAG TGA CCA GTT CAT CAG ATT CAT C-3′ R: 5′-CTA GGC ATC ACC TGT GCC ATA CC-3′	374bp
p16	F: 5′-GAC ATC CCC GAT TGA AAG AA-3′ R: 5′-TTT ACG GTA GTG GGG GAA GG-3′	198 bp
p21	F: 5′-GAC ACC ACT GGA GGG TGA CT-3′ R: 5′-CAG GTC CAC ATG GTC TTC CT-3′	172 bp
p53	F: 5′-GGC CCA CTT CAC CGT ACT AA-3′ R: 5′-GTG GTT TCA AGG CCA GAT GT-3′	156 bp