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. 2011 Nov 15;10(22):3938–3947. doi: 10.4161/cc.10.22.18107

Figure 5.

Figure 5

Cisplatin modulated cell viability via p-ΔNp63α/miR-885-3p axis. SCC-wt-ΔNp63α cells (A) and SCC-ΔNp63α-S385G cells (B) were transfected with the miR-885-3p inhibitor (curve 3) or miR-885-3p mimic (curve 4) for 24 h. Cells were then exposed to control medium (Con, curves 1 and 4) or 10 µg/ml cisplatin (CIS, curves 2 and 3) for an additional 0–120 h. Cell survival was monitored at 24, 48, 72, 96 and 120 h by measuring the mitochondrial activity by MTT reagent. Experiments were performed in triplicate with +SD as indicated (p < 0.05). In parallel experiments, SCC-wt-ΔNp63α cells were transfected with Mdm4 siRNA, pCMV6-Akt1, pCMV6-Bcl2 and pCMV6-Ulk2 (C), while SCC-ΔNp63α-S385G cells were transfected with pCMV6-Mdm4, siRNA against Akt1, Bcl2 or Ulk2 (D) for 24 h. Cells then were exposed to control medium or 10 µg/ml cisplatin for an additional 48 h. Cell viability was monitored at 72 h by measuring the mitochondrial activity by MTT reagent. Experiments were performed in triplicate with + SD, as indicated (p < 0.05).