Figure 14.

Histograms illustrating the quantity of CRH immunostaining in 5 levels of the PVN and in 4 levels of the central nucleus of the amygdala of the HSR, MSR and SS groups. Top left panel, shows the overall average CRH positive area (μm²)/section in each group. There was a significant difference between the groups with MSR and SS groups exhibiting significantly higher expression of CRH protein than the HSR group Top right panel shows the volume of CRH immunostaining expressed as a percent of the volume of the region (μm³). There was a significant difference between the groups with MSR and SS groups exhibiting significantly higher expression of CRH protein than the HSR group. Bottom panel illustrates the density of CRH fibers (positive pixel area covered by fibers) in the central nucleus of the amygdala from HSR, MSR and SS groups. There was a significant difference between the groups with the SS group exhibiting significantly higher CRH fiber density than the MSR and HSR groups * different from HSR; + different from MSR; ANOVA followed by Student-Newman-Keuls posthoc pairwise comparison. Reprinted from Centeno et al., 2007c.