Table 5.

Stages of DAPI-stained nuclear behavior in the control and cold-shocked eggs of the loach at specific time intervals after fertilization.

Stages	Time elapsed after fertilization (min)
	Control						Cold-shock
	10	20	30	40	50	60	10	30	60	70	80	90	100	110	120	130
1 condensed nucleus	0	0	0	0	0	0	5	3	6	11	6	6	1	4	1	0
2 condensed nuclei	4	0	0	0	0	0	12	16	15	5	11	4	3	1	1	0
3 condensed nuclei	4	0	0	0	0	0	3	0	0	8	3	0	1	0	0	0
2 condensed nuclei + 1 decondensed pronucleus	0	0	0	0	0	0	0	0	0	2	2	0	0	0	1	0
1 condensed nucleus + 1 decondensed pronucleus	0	0	0	0	0	0	0	0	0	0	5	5	1	0	0	0
2 decondensed pronuclei	0	6	1	0	0	0	0	0	0	0	2	1	0	0	0	0
1 decondensed pronucleus	0	3	4	1	1	0	0	0	0	0	2	3	1	0	1	0
First cleavage Anaphase	0	0	0	4	1	0	0	0	0	0	0	0	1	0	0	0
2-cell stage	0	0	0	0	1	11	0	0	0	0	0	0	0	0	3	9
Others 1	0	0	2	2	4	0	0	1	0	0	1	6	6	16	5	0
Total number of eggs observed	8	9	7	7	7	11	20	20	21	26	32	25	14	21	12	9

¹ cytological observation could not be made.