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. 2012 Jan 25;7(1):e29876. doi: 10.1371/journal.pone.0029876

Figure 2. Effect of 24 h UO on renal HIF-1α/2α expression.

Figure 2

WT mice were exposed to either sham-operation or left UO, which continued for 24 hours, and then was released. The left kidneys were harvested immediately (d0), 2, 4 or 7 days after release of obstruction (n = 4 at each time point). Immunoblot analyses of HIF-1α and HIF-2α in left kidneys were then performed and co-detection of TBP was performed to assess equal loading. HIF protein bands were quantified and normalized to TBP. Data were expressed as mean ± SD, and the mean value obtained from non-operated control mice was arbitrarily defined as 1. *, p<0.05 versus sham-operated controls; **, p<0.05 versus all the other groups. C, non-operated controls; S, sham-operated controls.