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. 2012 Jan 25;7(1):e30090. doi: 10.1371/journal.pone.0030090

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Hwang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A–F show the dorsal cluster of multidendritic neurons of larval abdominal segments immunostained with anti-GFP antibody (green) and anti-HRP (magenta). (A) Anti-GFP staining of pain^Gal4 UAS-Painless^p103::VFP larva. (B) Anti-HRP staining of pain^Gal4 UAS-Painless^p103::VFP. (C) Merge of A and B. (D) Anti-GFP staining of pain^Gal4 UAS-Painless^p60::VFP line 1. (E) Anti-HRP staining of pain^Gal4 UAS-Painless^p60::VFP line 1. (F) Merge of D and E. Note that the exposure times for acquisition of Painless^p60::VFP signal was significantly longer than for Painless^p103::VFP.