Table 1. Primer sequences for quantitative real-time PCR.
| Genes | Primer sequences (5′-3′) | T ann a (°C) |
| Nlrp2 | Fb: AACACTGAGCCTGAAACACTTGGA | 60 |
| Rc: CAGTTCAGTGGAGTGATGGAGCA | ||
| Nlrp4f | F: CTTGAACCAGGCAGAGTGCAAC | 60 |
| R: TGCCAAATTAAGAACCTTCAACGAC | ||
| Nlrp5 | F: CCAGAGCAGGAGCAGACATC | 60 |
| R: TTCCAATCCACGTGCTTTCA | ||
| Nlrp9c | F: CGCAATTGTACACACATTATCCAAG | 60 |
| R: TCAGGCATATAAGTGTTGGTCTCAG | ||
| Nlrp14 | F: TTCTCAGCGCAAGGACTTAACTTTC | 60 |
| R: AGTCTTTGTCCTCACTCACGGTTTC | ||
| β-actin | F: GAAGTGTGACGTTGACATCCG | 60 |
| R: ACTTGCGGTGCACGATGGAGG |
a
Annealing temperature.
b
Forward primer.
c
Reverse primer.