Table 1. Percentages of normal, affected, or dead embryos/larvae following microinjection with ex7:in7-MO or both MOs at different developmental stages.
Control | Ex7:in7-MO(21.4 ng) | Both MO(10.7 ng each) | ||
22 hpf | Normal (%) | 100 | 4.5 | 2.9 |
TCS-Phenotype (%) | 0 | 42.7 | 17.4 | |
Died (%) | 0 | 52.8 | 79.7 | |
TOTAL ( n ) | 58 | 89 | 105 | |
44 hpf | Normal (%) | 98.3 | 0 | 14.3 |
TCS-Phenotype (%) | 1.7 | 95.2 | 71.4 | |
Died (%) | 0 | 4.8 | 14.3 | |
TOTAL ( n ) | 58 | 42 | 21 | |
72 hpf | Normal (%) | 98.3 | 0 | 16.7 |
TCS-Phenotype (%) | 0 | 88.1 | 72.2 | |
Died (%) | 1.7 | 11.9 | 11.1 | |
TOTAL ( n ) | 58 | 40 | 18 |