Human |
MSP for ERα |
Unmethylated-specific pair |
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F: 5′-TGTTGTGTATAATTATTTTGAGGGT-3′
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R: 5′-CTCACACACCATATAACCACTAAAC-3′
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Methylated-specific pair |
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F: 5′-CGTCGTGTATAATTATTTCGAGGGC-3′
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R: 5′-CTCGCGCACCGTATAACCGCTAAAC-3′
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BSP for ERα |
First round pair |
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F: 5′-ATGGTTTTATTGTATTAGATTTAAGGGAAT-3′
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R: 5′-TATTACICTAAACTCITTCTCCAAATAATA-3′
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Second round pair |
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F: 5′-AGTGTATTTGGATAGTAGTAAG-3′
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R: 5′-CTAACCITAAAACTACAAAAAAAA-3′
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PCR for |
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DNMT1 |
F: 5′-GCACAAACTGACCTGCTTCA-3′
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R: 5′-GCCTTTTCACCTCCATCAAA-3′
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ERα |
F: 5′-TTCGGCTCCAACGGCCTGGGGGGTTT-3′
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R: 5′-GGTACTGGCCAATCTTTCTCTGCCACCCT-3′
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GAPDH |
F: 5′-GAAGGTGAAGGTCGGAGTC-3′
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R: 5′-GAAGATGGTGATGGGATTTC-3′
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SD rat |
MSP for ERα |
Unmethylated-specific pair |
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F: 5′-TGTTGTTGTTGTGTGGTTGTTGG-3′
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R: 5′-TCAACAAACTAAACAACACAC-3′
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Methylated-specific pair |
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F: 5′-TTAATTATTTCGAGGGCGTC-3′
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R: 5′-AAAACGACGACACGTACGAC-3′
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PCR for |
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DNMT1 |
F: 5′-ACCTACCACGCCGACAT-3′
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R: 5′-AGGTCCTCTCCGTACTCCA-3′
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ERα |
F: 5′-GGCTGCGCAAGTGTTACGAA-3′
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R: 5′-CATTTCGGCCTTCCAAGTCAT-3′
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GAPDH |
F: 5′-CCTTCATTGACCTCAACTAC-3′
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R: 5′-GGAAGGCCATGCCAGTGAGC-3′
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