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. 2012 Jan 25;7(1):e30635. doi: 10.1371/journal.pone.0030635

Table 1. Primer sets.

Species Gene Primer Sequence
Human MSP for ERα Unmethylated-specific pair
F: 5′-TGTTGTGTATAATTATTTTGAGGGT-3′
R: 5′-CTCACACACCATATAACCACTAAAC-3′
Methylated-specific pair
F: 5′-CGTCGTGTATAATTATTTCGAGGGC-3′
R: 5′-CTCGCGCACCGTATAACCGCTAAAC-3′
BSP for ERα First round pair
F: 5′-ATGGTTTTATTGTATTAGATTTAAGGGAAT-3′
R: 5′-TATTACICTAAACTCITTCTCCAAATAATA-3′
Second round pair
F: 5′-AGTGTATTTGGATAGTAGTAAG-3′
R: 5′-CTAACCITAAAACTACAAAAAAAA-3′
PCR for
DNMT1 F: 5′-GCACAAACTGACCTGCTTCA-3′
R: 5′-GCCTTTTCACCTCCATCAAA-3′
ERα F: 5′-TTCGGCTCCAACGGCCTGGGGGGTTT-3′
R: 5′-GGTACTGGCCAATCTTTCTCTGCCACCCT-3′
GAPDH F: 5′-GAAGGTGAAGGTCGGAGTC-3′
R: 5′-GAAGATGGTGATGGGATTTC-3′
SD rat MSP for ERα Unmethylated-specific pair
F: 5′-TGTTGTTGTTGTGTGGTTGTTGG-3′
R: 5′-TCAACAAACTAAACAACACAC-3′
Methylated-specific pair
F: 5′-TTAATTATTTCGAGGGCGTC-3′
R: 5′-AAAACGACGACACGTACGAC-3′
PCR for
DNMT1 F: 5′-ACCTACCACGCCGACAT-3′
R: 5′-AGGTCCTCTCCGTACTCCA-3′
ERα F: 5′-GGCTGCGCAAGTGTTACGAA-3′
R: 5′-CATTTCGGCCTTCCAAGTCAT-3′
GAPDH F: 5′-CCTTCATTGACCTCAACTAC-3′
R: 5′-GGAAGGCCATGCCAGTGAGC-3′