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. 2012 Jan 25;7(1):e30872. doi: 10.1371/journal.pone.0030872

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Flow cytometry was used to detect cell surface FV glycosylated gag using the monoclonal antibody mAb34 (Viral antigen). FDCs are B220⁺ cells gated for lack of expression of CD11c, CD8, CD19, CD4, CD3, CD11b and Gr-1, but staining positive for MHCII. The representative plot of FDCs from an acutely infected mouse shows no viral antigen staining above background (Naïve) levels.