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. 2012 Jan 25;7(1):e31120. doi: 10.1371/journal.pone.0031120

Figure 3. VHL-deficient cells are hypersensitive to ELR510444-induced apoptosis.

Figure 3

(A) HIF-2α is reduced in A498 and 786-O cells transfected with VHL. Cells were transfected with empty vector or VHL. Positively transfected cells were selected by incubation with puromycin. HIF-2α was measured by immunoblotting. (B) VEGF levels are reduced in the presence of VHL. Cells were plated and media was collected 16 h later. VEGF levels were measured by ELISA assay. Mean ± SD, n = 3. *Indicates a significant difference compared to controls, p<0.05. (C) A498/VHL and 786-O/VHL cells are less sensitive to ELR510444-mediated apoptosis. Cells were treated with 10 nM ELR510444 for 24 h and apoptosis was measured by PI-FACS analysis. Mean ± SD, n = 3. *Represents a significant difference compared to ELR510444-treated vector-only transfected cells, p<0.05.