Figure 2. Estrogen receptor ligands induced ERK1/2 phosphorylation in SUM149 and SUM190 cells.

(A–C) The cells were maintained in 5% DCC for 48 hours and then treated with E2 (10 nM), DPN (10 nM) or G1 (10 nM) for the indicated times and then ERK1/2 phosphorylation was analyzed by western blotting. The expression level of total ERK was analyzed for normalization. Error bars indicate standard deviation.