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. 2012 Jan 25;7(1):e30725. doi: 10.1371/journal.pone.0030725

Figure 4. Effect of MEK inhibitor U0126 on p-ERK1/2 activation and cytoskeletal remodeling induced by estrogen receptor ligands and antiestrogens.

Figure 4

(A) Blocking of E2, DPN, 4OHT or G1 induced ERK1/2 phosphorylation in SUM149 cells by U0126. The cells were maintained in 5% DCC for 48 hours and then pretreated with or without U0126 (20 µM) for 15 min before treatment with or without E2 (10 nM), DPN (10 nM), 4OHT (10 nM) and G1 (10 nM) for 5 min. ERK1/2 phosphorylation was analyzed by western blotting. (B) Blocking of E2-induced cytoskeletal change in SUM149 cells by U0126. The cells were maintained for 48 hours in 5% DCC and then pretreated with U0126 (20 µM) before the treatment with or without E2 (10 nM) for 5 min. The cells were fixed and labeled with fluorescently conjugated phalloidin (for filamentous actin, red) and DAPI (for DNA). White-dotted areas in upper panels were magnified in lower panels.