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Fluorescence spectra (λex = 494 nm) of (dark green) fluorescein-labeled pNIPMAm-BAC-Bis particles, (orange) unlabeled particles, (blue) pH 7.4 HEPES buffer, and (red) the supernatant following purification by centrifugation. Particles that were not reduced and therefore retained their disulfide linkages (light green) had much lower coupling efficiency than that of the reduced particles.
