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. 2003 Dec;2(6):1187–1199. doi: 10.1128/EC.2.6.1187-1199.2003

FIG. 3.

FIG. 3.

Constitutively active kpp4-2 and fuz7DD trigger pheromone gene expression. (A) Overexpression of pra2 does not rescue pheromone gene expression in prf1 deletion strains. The strains indicated on the top were treated for 5 h with synthetic a1 pheromone dissolved in DMSO (+) or with the same volume of DMSO (−). RNA was isolated, and 10 μg of total RNA was loaded per lane. The blot was probed with mfa2 and with rRNA as loading control. (B and C) Activation of the MAPK cascade elevates pheromone gene expression. FB1 and the FB1-derived strains indicated on the top were grown with glucose (−) or arabinose (+) as a carbon source. RNA was isolated, 15 μg of total RNA were loaded per lane and the same filters were hybridized in succession with the probes indicated on the right.