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. 2003 Dec;2(6):1187–1199. doi: 10.1128/EC.2.6.1187-1199.2003

FIG. 4.

FIG. 4.

Pheromone signaling via the Kpp4/Fuz7/Kpp2 cascade affects b gene expression. (A) FB1 and the FB1-derived strains indicated on the top were treated for 5 h with synthetic a2 pheromone dissolved in DMSO (+) or with DMSO (−). RNA was isolated, and 10 μg of total RNA was loaded per lane. Blots were hybridized with the probes indicated on the right. wt, wild type. (B) kpp2K50R encodes a kinase-dead Kpp2 protein. The GST fusion proteins indicated at the top were purified from E. coli and subjected to kinase assay with MBP as the substrate. The upper panel shows Coomassie blue staining, and the lower panel depicts the incorporated radioactive phosphate in MBP. (C) fuz7DD elevates b gene expression. FB1 and the FB1-derived strains indicated on the top were grown with glucose (−) or arabinose (+) as a carbon source. RNA was isolated, and 15 μg of total RNA was loaded per lane. The filter was hybridized in succession with the probes indicated on the right.