TABLE 3.
Plant infection assays
| Inoculum | No. of:
|
% Tumor formationb | |
|---|---|---|---|
| Infected plantsa | Plants with tumors | ||
| FB1 × FB2 | 40 | 36 | 90 |
| FB1Δkpp4 × FB2Δkpp4 | 155 | 0 | 0 |
| SG200 | 40 | 30 | 75 |
| SG200Δkpp4 | 117 | 0 | 0 |
| FB1kpp4WT × FB2kpp4WT | 44 | 38 | 86 |
| FB1kpp4RA × FB2kpp4RA | 37 | 15 | 40 |
| HA103 | 135 | 82 | 60 |
| HA103Δkpp4 | 156 | 0 | 0 |
| FB1 × FB2 | 18 | 14 | 93 |
| FB1Δfuz7 × FB2Δfuz7 | 61 | 0 | 0 |
| SG200Δfuz7 | 42 | 0 | 0 |
| HA103 | 38 | 32 | 84 |
| HA103Δfuz7 | 75 | 0 | 0 |
| FB1 × FB2 | 40 | 35 | 87 |
| SG200 | 64 | 58 | 91 |
| FB1kpp2-GFP × FB2kpp2-GFP | 77 | 60 | 78 |
| FB1Δkpp2-1 × FB2Δkpp2-1 | 77 | 16 | 20 |
| FB1kpp2WT × FB2kpp2WT | 40 | 38 | 95 |
| SG200kpp2WT | 40 | 37 | 80 |
| FB1kpp2AEF × FB2 | 36 | 18 | 50 |
| FB1kpp2AEF × FB2kpp2AEF | 76 | 2c | 3 |
| SG200kpp2AEF | 115 | 0 | 0 |
a
All infections were performed twice with two independently generated mutants.
b
Percentage of plants that developed at least one tumor on a stem or leaf.
c
Tumors observed were found on leaves only and did not extend 2 mm in diameter. This is significantly different from infections with wild-type strains, where tumors develop on all green parts of the plant and reach diameters of up to 50 mm.