FIG. 2.

Leu treatment impairs β-cell differentiation. A: Leu did not modify the global growth of E13.5 rat pancreata that were cultured at the air medium/interface for varying durations (day [D]1 to D7). B: Immunohistochemical analyses of E13.5 pancreata after 7 days in culture, with and without Leu treatment. Acinar cell and β-cell development were evaluated using an antibody against amylase (green) and an antibody against insulin (red), respectively. Nuclei were stained with Hoechst 33342 fluorescent stain (blue). Scale bar = 50 μm. C: Absolute areas that were occupied by the nuclei and the amylase- and insulin-positive cells were quantified using NIH Image J software. Data are mean ± SEM from at least three pancreata per condition. NS, not significant; **P < 0.01. (A high-quality digital representation of this figure is available in the online issue.)