FIG. 3.

Leu treatment impairs β-cell differentiation mainly by causing a loss of NGN3 expression. A: Real-time PCR quantification of Ngn3, insulin, and amylase mRNA in cultured E13.5 pancreatic explants that were treated with Leu (black bars) or not treated with Leu (white bars) after 0, 1, 3, 5, and 7 days. B and C: Immunodetection and quantification of NGN3-positive cells from pancreata that were cultured for 3 days with or without Leu. Scale bar = 100 μm. Data are mean ± SEM of at least three independent experiments. NS, not significant; *P < 0.05, **P < 0.01, ***P < 0.005. (A high-quality digital representation of this figure is available in the online issue.)