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. 2003 Dec;2(6):1178–1186. doi: 10.1128/EC.2.6.1178-1186.2003

FIG. 5.

FIG. 5.

Effects of the overexpression of aflR on ST biosynthesis in the A. nidulans veA+ and ΔveA strains. (A) The expression of aflR and stcU in FGSC4 (veA+), RNKT1 (ΔveA), RNKT6 [alcA(p)::aflR veA+], and RNKT4 [alcA(p)::aflR ΔveA] strains was evaluated by mRNA analysis. Expression of the alcA(p)::aflR construct results in an aflR functional transcript slightly larger than the endogenous transcript (24). Total RNA was isolated from the cultures at zero h and 24 h after transfer from liquid GMM into liquid TMM. rRNA stained with ethidium bromide is shown to indicate RNA loading. (B) ST analysis by TLC. The uncharacterized compound observed in ΔveA near the ST Rf is not ST (in this assay, ST fluoresces bright yellow while the unknown compound fluoresced blue). Std, standard.