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In vitro bioactivity of VEGF loaded in col-PCL scaffold. HUVEC density was visualized by live-dead stain after 72 hours of culture in absence of scaffolds, in presence of col-PCL without VEGF or, in presence of col-PCL loaded with 1 μg of VEGF (A). Col-PCL loaded with 1 μg of VEGF and without VEGF were placed on chicken embryo CAM (E8) and assessed for blood vessel growth around scaffolds after 48 hours incubation (B).
