Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Gene Ther. 2011 Oct 20;19(5):576–581. doi: 10.1038/gt.2011.138

FIG. 2.

FIG. 2

FIG. 2

FIG. 2

Immunostaining and current tracing of TA BAAV-CFTRΔR transduced CF HAE. Immunostaining of differentiated airway epithelia expressing BAAV-CFTRΔR (10×106 genomes (gc)/cell) (panels A-C) or ad5-CFTRΔR (100MOI) (D-F). Data are X-Y confocal images (B-C and E-F) or en face images (A and D). CFTR is shown in green, tight junction protein zonula occludens-1 (ZO-1) in red and indicated by arrows of the same color respectively. DAPI nuclear stain is in blue. (G) Current tracings of CF airway epithelia transduced with the indicated amounts of BAAV-173CMV-CFTRΔR (106 or 104 gc/cell). Millicells were treated sequentially with, amiloride (10−4M), forskolin (10−4M)/IBMX (10−5M), and bumetanide (10−4M) as indicated. Asterisk indicates a change in the recorder scale. (H) Bumetanide-sensitive forskolin-stimulated current (in μamps/cm2) in well-differentiated CF epithelia expressing the indicated amount of BAAV-CFTRΔR at 7 or 21 days post-transduction. N=3 for each AAV viral titer; N=1 for ad5-GFP control at 7 and 21 day time points. One-way ANOVA with Bonferroni post test * (P value <0.05).