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. Author manuscript; available in PMC: 2012 Nov 1.

Published in final edited form as: Gene Ther. 2011 Oct 20;19(5):576–581. doi: 10.1038/gt.2011.138

FIG. 3 — Lung TA mediated BAAV-Luc transduction in vivo.

(A and B) BAAV-Luc (5^.10⁹ DRP) was administered to the lungs of 2-5 mice as indicated for each TA concentration tested, by nasal instillation. Transduction was imaged (A) 24 hrs later using a Xenogen camera 15 min following administration of luciferin substrate. (B) Luciferase activity was quantified 24 to72 hrs post BAAV administration, by Living Image (R), version 2.60.1. Medians represented by horizontal lines. Student’s t-test * P value <0.05. (C) BAAV or AAV2-Luc (5^.10⁹ DRP) was delivered to the lungs of mice via an intranasal route with or without chitotriose (20mg/ml). In the indicated mice, prior to vector delivery, mice were treated with TA as described above. Transduction was imaged 24 hrs later using a Xenogen camera 15 min following injection of luciferin.

(D) Lungs of mice were removed 4 days post-transduction, inflated with agarose prior to imbedding in paraffin. Luciferase positive cells were detected by immunostaining and pseudocolored in green (left panel). The bright field image is shown on the right. Original magnification 63X.

FIG. 3 — Lung TA mediated BAAV-Luc transduction in vivo.

(A and B) BAAV-Luc (5^.10⁹ DRP) was administered to the lungs of 2-5 mice as indicated for each TA concentration tested, by nasal instillation. Transduction was imaged (A) 24 hrs later using a Xenogen camera 15 min following administration of luciferin substrate. (B) Luciferase activity was quantified 24 to72 hrs post BAAV administration, by Living Image (R), version 2.60.1. Medians represented by horizontal lines. Student’s t-test * P value <0.05. (C) BAAV or AAV2-Luc (5^.10⁹ DRP) was delivered to the lungs of mice via an intranasal route with or without chitotriose (20mg/ml). In the indicated mice, prior to vector delivery, mice were treated with TA as described above. Transduction was imaged 24 hrs later using a Xenogen camera 15 min following injection of luciferin.

(D) Lungs of mice were removed 4 days post-transduction, inflated with agarose prior to imbedding in paraffin. Luciferase positive cells were detected by immunostaining and pseudocolored in green (left panel). The bright field image is shown on the right. Original magnification 63X.